Steroids are normally prescribed by doctors to HIV and cancer patients to help them gain a healthy weight. These diseases normally cause drastic weight loss in patients, so steroids can help them gain a healthy weight. If you are underweight, you can use steroids as a weight gain solution. It is important to note that you cannot gain weight by simply using steroids, you have to provide the body with the nutrients needed to build cells and tissues. Therefore, you must eat well. Be sure to increase the protein and carb portions in your meals. This will go a long way in increasing your weight once steroids have changed your physiological processes to pave way for weight gain.
Steroid isolation , depending on context, is the isolation of chemical matter required for chemical structure elucidation, derivitzation or degradation chemistry, biological testing, and other research needs (generally milligrams to grams, but often more  or the isolation of "analytical quantities" of the substance of interest (where the focus is on identifying and quantifying the substance (for example, in biological tissue or fluid). The amount isolated depends on the analytical method, but is generally less than one microgram.  [ page needed ] The methods of isolation to achieve the two scales of product are distinct, but include extraction , precipitation, adsorption , chromatography , and crystallization . In both cases, the isolated substance is purified to chemical homogeneity; combined separation and analytical methods, such as LC-MS , are chosen to be "orthogonal"—achieving their separations based on distinct modes of interaction between substance and isolating matrix—to detect a single species in the pure sample. Structure determination refers to the methods to determine the chemical structure of an isolated pure steroid, using an evolving array of chemical and physical methods which have included NMR and small-molecule crystallography .  : 10–19 Methods of analysis overlap both of the above areas, emphasizing analytical methods to determining if a steroid is present in a mixture and determining its quantity.